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Becton Dickinson live cell (dapi) sorter
$<t>Neuronal</t> cells detach from standard glass surfaces sooner than from tissue culture polystyrene (A) Schematic of the cell culture microenvironment and modifications tested to improve neuronal attachment. (B) Human neural progenitor cells (NPCs) were pre-matured in BrainPhys + supplements for 0–2 weeks, replated on test surfaces, and further matured for up to 27 weeks in BrainPhys + supplements before phenotypic analyses. Before replating on test surfaces, post-mitotic precursors were sorted for <t>DAPI</t> exclusion at 2 weeks (see Experimental procedures). (C and D) Live neuronal cultures under phase contrast at 4× and 20× magnification, respectively. Detachment was quantified from 4× magnification phase contrast images by tracing regions with no cells (yellow) and calculating their combined area as a fraction of the total field of view area for each well. (E) Detachment on standard glass across multiple coating solutions: poly- l -ornithine (PLO; n = 4), PLO-laminin (Lam) (n = 6), Matrigel (n = 2), 24 h treatment of laminin (n = 6), 4 h treatment of laminin (n = 2), uncoated (n = 6), and poly- l -lysine (PLL; n = 2); n replicates from 2 independent experiments, means ± SEMs represented. (F) Cell detachment on standard glass compared to plastic (TCPS) both with PLO-Lam. Means ± SEMs from n = 5 biologically independent experiments, 2–6 replicates per experiment. (G and H) Spontaneous multi-electrode array (MEA) recordings of hPSC-derived neuronal cultures showing network burst activity and synchrony over 15–17 weeks on TCPS plates pre-coated with PLO-Lam. Means ± SEMs data from 16 replicate wells, each well containing 16 electrodes.$
Live Cell (Dapi) Sorter, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/live+cell+(dapi)+sorter/live+cell++dapi++sorter/pmc09039832-276-19-22
Average 90 stars, based on 1 article reviews

live cell (dapi) sorter - by Bioz Stars, 2026-10

90/100 stars

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1) Product Images from "Long-term adherence of human brain cells in vitro is enhanced by charged amine-based plasma polymer coatings"

Article Title: Long-term adherence of human brain cells in vitro is enhanced by charged amine-based plasma polymer coatings

Journal: Stem Cell Reports

doi: 10.1016/j.stemcr.2022.01.013

$Neuronal cells detach from standard glass surfaces sooner than from tissue culture polystyrene (A) Schematic of the cell culture microenvironment and modifications tested to improve neuronal attachment. (B) Human neural progenitor cells (NPCs) were pre-matured in BrainPhys + supplements for 0–2 weeks, replated on test surfaces, and further matured for up to 27 weeks in BrainPhys + supplements before phenotypic analyses. Before replating on test surfaces, post-mitotic precursors were sorted for DAPI exclusion at 2 weeks (see Experimental procedures). (C and D) Live neuronal cultures under phase contrast at 4× and 20× magnification, respectively. Detachment was quantified from 4× magnification phase contrast images by tracing regions with no cells (yellow) and calculating their combined area as a fraction of the total field of view area for each well. (E) Detachment on standard glass across multiple coating solutions: poly- l -ornithine (PLO; n = 4), PLO-laminin (Lam) (n = 6), Matrigel (n = 2), 24 h treatment of laminin (n = 6), 4 h treatment of laminin (n = 2), uncoated (n = 6), and poly- l -lysine (PLL; n = 2); n replicates from 2 independent experiments, means ± SEMs represented. (F) Cell detachment on standard glass compared to plastic (TCPS) both with PLO-Lam. Means ± SEMs from n = 5 biologically independent experiments, 2–6 replicates per experiment. (G and H) Spontaneous multi-electrode array (MEA) recordings of hPSC-derived neuronal cultures showing network burst activity and synchrony over 15–17 weeks on TCPS plates pre-coated with PLO-Lam. Means ± SEMs data from 16 replicate wells, each well containing 16 electrodes.$
Figure Legend Snippet: Neuronal cells detach from standard glass surfaces sooner than from tissue culture polystyrene (A) Schematic of the cell culture microenvironment and modifications tested to improve neuronal attachment. (B) Human neural progenitor cells (NPCs) were pre-matured in BrainPhys + supplements for 0–2 weeks, replated on test surfaces, and further matured for up to 27 weeks in BrainPhys + supplements before phenotypic analyses. Before replating on test surfaces, post-mitotic precursors were sorted for DAPI exclusion at 2 weeks (see Experimental procedures). (C and D) Live neuronal cultures under phase contrast at 4× and 20× magnification, respectively. Detachment was quantified from 4× magnification phase contrast images by tracing regions with no cells (yellow) and calculating their combined area as a fraction of the total field of view area for each well. (E) Detachment on standard glass across multiple coating solutions: poly- l -ornithine (PLO; n = 4), PLO-laminin (Lam) (n = 6), Matrigel (n = 2), 24 h treatment of laminin (n = 6), 4 h treatment of laminin (n = 2), uncoated (n = 6), and poly- l -lysine (PLL; n = 2); n replicates from 2 independent experiments, means ± SEMs represented. (F) Cell detachment on standard glass compared to plastic (TCPS) both with PLO-Lam. Means ± SEMs from n = 5 biologically independent experiments, 2–6 replicates per experiment. (G and H) Spontaneous multi-electrode array (MEA) recordings of hPSC-derived neuronal cultures showing network burst activity and synchrony over 15–17 weeks on TCPS plates pre-coated with PLO-Lam. Means ± SEMs data from 16 replicate wells, each well containing 16 electrodes.

Techniques Used: Cell Culture, Derivative Assay, Activity Assay

DAP-treated glass surface supports human proliferative neuronal/glia progenitors, brain tumor cells, and post-mitotic mature neurons and astrocytes (A–D) Analysis of proliferative human brain cells on various substrates. Attachment determined by counting fixed nuclei (DAPI + ) over time. Means ± SEMs fold change in number of nuclei per well (normalized to day 0) calculated for 5–6 replicate wells/condition/time point (1 independent experiment). All of the surfaces were pre-coated with Matrigel. Proliferative human brain cells analyzed were (A) patient-derived glioblastoma tumor cells (GBMs), (B) hESC-derived glial precursor cells (GPCs), and (C) hESC-derived neural progenitor cells (NPCs). (D) Immunofluorescence staining of DNA (DAPI; blue) and cell soma (CellTracker, red) at latest time point (8 days for brain cells). See also <xref ref-type=

Figure S3 . (E) Proliferation of non-neuronal human embryonic stem cells (H9, hESC) was measured as the fraction of area covered by colonies normalized to day 0 (n = 5–6 replicates from 1 independent experiment). (F) Immunofluorescence staining of DNA (DAPI; blue) and cell soma (CellTracker, red) at latest time point (5 days for stem cells). See also Figure S3 . (G and H) Cytotoxicity analysis of neuronal cultures. All of the surfaces were pre-coated with PLO-Lam. (G) Lactate dehydrogenase (LDH) relative concentrations measured in neuronal culture supernatant (n = 15 replicates from 5 independent experiments) to estimate cellular stress. (H) Without fixation, DNA marker DAPI is incorporated in the nuclei of dead or dying cells (DAPI + ), but not live cells (DAPI − ). Means ± SEMs proportions of live cells (DAPI − ). (I) Immunofluorescence staining of MAP2, GFAP, and synapsin at 10× magnification (top) and 40× magnification (bottom) in neuronal cultures after 9 weeks in maturation medium, on glass-DAP-LAM. (J) Live neuronal cultures on glass-DAP-LAM under phase contrast at 20× after 27 weeks in maturation medium. (K) FACS analysis of the proportion of neurons (Lv-synapsin-GFP) and astrocytes (Lv-GFAP-tdTomato) at 9 weeks in maturation medium on different surfaces. For (H) and (K), n = 3–5 replicates per condition from 2 independent experiments. p values calculated using Mann-Whitney tests, ns for p > 0.05. " title="... various substrates. Attachment determined by counting fixed nuclei (DAPI + ) over time. Means ± SEMs fold ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: DAP-treated glass surface supports human proliferative neuronal/glia progenitors, brain tumor cells, and post-mitotic mature neurons and astrocytes (A–D) Analysis of proliferative human brain cells on various substrates. Attachment determined by counting fixed nuclei (DAPI + ) over time. Means ± SEMs fold change in number of nuclei per well (normalized to day 0) calculated for 5–6 replicate wells/condition/time point (1 independent experiment). All of the surfaces were pre-coated with Matrigel. Proliferative human brain cells analyzed were (A) patient-derived glioblastoma tumor cells (GBMs), (B) hESC-derived glial precursor cells (GPCs), and (C) hESC-derived neural progenitor cells (NPCs). (D) Immunofluorescence staining of DNA (DAPI; blue) and cell soma (CellTracker, red) at latest time point (8 days for brain cells). See also Figure S3 . (E) Proliferation of non-neuronal human embryonic stem cells (H9, hESC) was measured as the fraction of area covered by colonies normalized to day 0 (n = 5–6 replicates from 1 independent experiment). (F) Immunofluorescence staining of DNA (DAPI; blue) and cell soma (CellTracker, red) at latest time point (5 days for stem cells). See also Figure S3 . (G and H) Cytotoxicity analysis of neuronal cultures. All of the surfaces were pre-coated with PLO-Lam. (G) Lactate dehydrogenase (LDH) relative concentrations measured in neuronal culture supernatant (n = 15 replicates from 5 independent experiments) to estimate cellular stress. (H) Without fixation, DNA marker DAPI is incorporated in the nuclei of dead or dying cells (DAPI + ), but not live cells (DAPI − ). Means ± SEMs proportions of live cells (DAPI − ). (I) Immunofluorescence staining of MAP2, GFAP, and synapsin at 10× magnification (top) and 40× magnification (bottom) in neuronal cultures after 9 weeks in maturation medium, on glass-DAP-LAM. (J) Live neuronal cultures on glass-DAP-LAM under phase contrast at 20× after 27 weeks in maturation medium. (K) FACS analysis of the proportion of neurons (Lv-synapsin-GFP) and astrocytes (Lv-GFAP-tdTomato) at 9 weeks in maturation medium on different surfaces. For (H) and (K), n = 3–5 replicates per condition from 2 independent experiments. p values calculated using Mann-Whitney tests, ns for p > 0.05.

Techniques Used: Derivative Assay, Immunofluorescence, Staining, Marker, MANN-WHITNEY

FACS:

Article Title: Mammary Epithelial Cell Lineage Changes During Cow's Life.
Article Snippet: Milk production is highly dependent on the optimal development of the mammary epithelium.. It is therefore essential to better understand mammary epithelial cell growth and maintenance from the related epithelial lineage during the animal life.. Here, we characterized the epithelial lineage at puberty, lactation and dry-off in bovine using the cell surface markers CD49f, CD24, and CD10.

Article Title: Single-cell analysis reveals a hair follicle dermal niche molecular differentiation trajectory that begins prior to morphogenesis
Article Snippet: Single-cell dissociation Embryonic dorsolateral/flank skin was micro-dissected and pooled (3-4 embryos per sample) and dissociated into a single-cell suspension using 0.25% trypsin (Gibco, Life Technologies) for 30 minutes at 37° C. Single-cell suspensions we re then stained with DAPI (Fisher Scientific, NBP2-31156) just prior to fluorescence-activated cell sorting. .. Fluorescence-activated cell sorting DAPI-excluded live skin cells were sorted on a BD Facs Aria II (Biosciences) sorter with a 100 μm nozzle. .. Cells were sorted in bulk for 10X Genomics platform.

Article Title: Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells
Article Snippet: Statistical analysis was performed using ANOVA followed by a Scheffe test to for significance among individual groups (Systat v13). .. All cells were dissociated using accutase (Stem cell technologies) for thirty minutes, then resuspended in Neurobasal/B27 with Y27632 (10μM; Tocris) and sorted on a BD FACS Aria II (SSC gating/FSC gating/DAPI negative exclusion gating for live cells). .. For post-sort analysis, data was processed using FlowJo (Treestar) software.

Article Title: Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells
Article Snippet: Statistical analysis was performed using ANOVA followed by a Scheffe test to for significance among individual groups (Systat v13). .. FACS All cells were dissociated using accutase (Stem cell technologies) for thirty minutes, then resuspended in Neurobasal/B27 with Y27632 (10μM; Tocris) and sorted on a BD FACS Aria II (SSC gating/FSC gating/DAPI negative exclusion gating for live cells). .. For post-sort analysis, data was processed using FlowJo (Treestar) software.

Article Title: Bovine mammary gland development: new insights into the epithelial hierarchy
Article Snippet: .. Single live cells were gated by DAPI exclusion and sorted on a BD FACS ARIA II flow cytometer (BIOSIT CytomeTRI technical Platform – Villejean Campus, Rennes, France). ..

Flow Cytometry:

Article Title: Progenitor-derived endothelin controls dermal sheath contraction for hair follicle regression.
Article Snippet: Substantial follicle remodelling during the regression phase of the hair growth cycle is coordinated by the contraction of the dermal sheath smooth muscle, but how dermal-sheath-generated forces are regulated is unclear.. Here, we i de nt ify s pa ti ot em po rally controlled endothelin signalling—a potent vasoconstriction-regulating pathway—as the key activating mechanism of dermal sheath contraction.. Pharmacological blocking or genetic ablation of both endothelin receptors, ETA and ETB, impedes dermal sheath contraction and halts follicle regression.

Staining:

Article Title: Ketogenic diet modifies ribosomal protein dysregulation in KMT2D Kabuki syndrome
Article Snippet: .. PBMCs were stained with DAPI and sorted into a single cell suspension of live cells using the BD FACSAriaTM III Cell Sorter. ..

Single Cell:

Suspension:

Fluorescence:

other:

Article Title: Brain dendritic cells in ischemic stroke: time course, activation state, and origin
Article Snippet: Dead cells were excluded with DAPI and live cells were evaluated using a BD LSR-II FACS analyzer using FACS Diva software.

Review